Flow Cytometry
Overview
The Flow Cytometry Core Facility is a centralized service for Moffitt Cancer Center members with cancer-related research interests. Flow cytometry is an essential analytical tool for several Cancer Center projects due to its versatility and usefulness for rapid and simultaneous analysis of multiple characteristics on large numbers of cells, as well as for the identification of different cell types in mixed populations. Another fundamental use of flow cytometry instrumentation is for the isolation and purification of cells of a specific characteristic(s) from a heterogeneous population, which allows investigators the ability to dissect the functional and molecular properties of unique target populations.
Some specialized cancer research applications utilizing flow cytometry include analysis of DNA content and cell cycle status, assessment of apoptosis, phenotyping subpopulations, and detection of various signal transduction events. Flow cytometry is a diverse analytical technique that is widely used in clinical and basic research pursuits to understand cancer biology and control. The field of Flow Cytometry is ever expanding to accommodate the needs of investigators, as well as pioneering novel technology to answer complex scientific questions.