Cancer Pharmacokinetics and Pharmacodynamics

Purpose

The Cancer Pharmacokinetics and Pharmacodynamics Core (CPPC) goal is to facilitate members' efforts to discover, refine and progress new medicines by providing state-of-the-art pharmacokinetic and pharmacodynamic services in both preclinical and clinical settings. Specific aims are to support early drug discovery and lead optimization programs via absorption, distribution, metabolism and excretion (ADME)/PK studies to identify candidates for drug development and commercialization; to provide clinical pharmacokinetic and pharmacodynamic evaluations in support of Moffitt CCSG programs; and to provide collaborative design and education to members and staff, emphasizing the importance and practice of pharmacologic evaluations in cancer research.

Services

Pharmacokinetic Services
Drug Quantitation via Liquid Chromatography Mass Spectrometry (LC-MS/MS)
                  Clinical Research
                  Pre-clinical (murine)
                  Basic Science (cell lines)     
In Vitro ADME 
                  Compound Stability (Microsomal, Plasma, Chemical)
                  Enzyme Inhibition Studies
                  Compound Solubility (Kinetic)
                  Permeability (PAMPA)
Pharmacokinetic Analysis
                  Non-Compartmental & Compartmental Analysis
Pharmacodynamic Services
Molecular Endpoint Analysis
                  Hematologic Subset Enrichment
                  Flow/PhosFlow Setup
                  Western Blotting/EMSA
                  ELISA and CBA/Luminex Setup
Drug Screening/Drug Interaction Analysis
                  384- and 1536-well Plate - Cell Based Assays
                  Viability/Cytotoxicity/Apoptosis IC50 Analysis
                  Drug Synergy/Antagonism Analysis (EoHSA/CI)

Please contact the Core by phone or email for more details on current services and pricing.   

Scientific Directors:  
Andrii Monastyrskyi, PhD
andrii.monastyrskyi@moffitt.org
Office: 813-745-3660

Manager, Translational Sciences Shared Resources:
Anthony Neuger, MS
anthony.neuger@moffitt.org
813-745-2288

Core Facility Manager:
Samer Sansil, MS
samer.sansil@moffitt.org
813-745-5759

Core Staff:
Staff Scientist – Ramu Kakumanu, MS
Research Specialist - Ashley McKee, MS
Research Specialist - Alicia Castaneda, BS

Location
The Cancer Pharmacokinetics and Pharmacodynamics Core is located in the Moffitt Research Center (MRC) on the first floor, rooms 1304 & 1305.

Scheduling
The Cancer Pharmacokinetics and Pharmacodynamics Core uses a Laboratory Information Management Suite (LIMS) located on the Moffittnet for billing and usage tracking.

Publications

All publications based on work conducted in the Cancer Pharmacokinetics and Pharmacodynamics Core should acknowledge the facility. A suggested statement is as follows:

This work has been supported in part by the Cancer Pharmacokinetics and Pharmacodynamics Core at the H. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute and funded in part by Moffitt’s Cancer Center Support Grant (P30-CA076292)

If a Core Staff member(s) significantly contributes or participates in a project, investigators are encouraged to consider including the individual(s) in the list of authors.