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Shared Resources

Directed by Dr. Brian Ruffell, the Flow Cytometry Core provides Moffitt members with access to state-of-the-art instrumentation and cutting-edge flow cytometry applications. The services that are provided by this Core include: (1) multi-parameter single-cell analyses, including cell cycle, apoptosis, viability, cell proliferation, ploidy, phenotyping, fluorescent proteins, signal transduction, bead array kinetics, cytokines, exosomes, and morphology; (2) cell sorting; (3) courses and workshops; (4) instrument training and project consultation; and 5) experiment and panel design.


  • Multi-parameter analysis including DNA cell cycle, ploidy, apoptosis, viability, phenotyping, kinetics, cytokines, fluorescent proteins, proliferation, signal transduction, bead array, and morphology.
  • Cell sorting
  • Data analysis and graphics
  • Publication-quality documents
  • Instrument training and project consultation
  • Courses and workshops
  • Current literature base including cytometry journals, manufacturer information on instrumentation, methodology, protocols, techniques, and the latest vendor products and services.


  • LSRII Flow Cytometer (2)
  • FACSymphony A3
  • FACSymphony A5
  • Aurora Spectral Cytometer
  • FACSCanto II Flow Cytometer
  • MACSQuant VYB Flow Cytometer
  • ImageStreamX Mark II Image Cytometer
  • FACSAria SORP Cell Sorter (2)
  • Sony SH800 Cell Sorter
  • MACSQuant Tyto Cell Sorter
  • Luminex100 Multiplex Bead Array
  • autoMACS Pro Magnetic Sorter
  • FlowJo; FCS Express; FluoroFinder

Hours of Operation
The Flow Cytometry Core is open 24 hours a day, seven days a week. Core Staff are available for assisted services Monday-Friday, 9 a.m.-6 p.m. Assisted after-hours usage must be scheduled in advance and approved by the Core Facility Manager. After hours, unassisted usage of the analyzers is available for trained and approved users. Usage is on a first-come, first-served basis; however, priority is given to Moffitt Cancer Center members with peer-reviewed funded projects.

The Flow Cytometry Core is located in the Moffitt Research Center (MRC) on the first floor, room 1295.  

The FCC uses a Laboratory Information Management Suite (LIMS) located on the Moffittnet for all Core requests, scheduling, billing, and usage tracking.

All publications based on work conducted in the Flow Cytometry Core Facility should acknowledge the facility.  A suggested statement is as follows:

This work has been supported in part by the Flow Cytometry Core at the H. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute and funded in part by Moffitt’s Cancer Center Support Grant (P30-CA076292)

If a Core Staff member(s) significantly contributes or participates in a project, investigators are encouraged to consider including the individual(s) in the list of authors.

Scientific Director
Brian Ruffell, PhD

Core Facility Manager  
Jodi Kroeger     
Office: 813-745-6705
Lab: 813-745-2005